The lyophilized hydrolysate (1 g), with a DH of 6.6%, obtained by
treatment with Alcalase for 15 min, was suspended in 5 ml of
distilled water, then separated onto a Sephadex G-25 gel filtration
column (5.2 56 cm) pre-equilibrated and eluted with distilled
water. Fractions (4 ml) were collected at a linear flow rate of 30 ml/
h. Fractions (4 ml each) were collected and elution curves were
obtained by measuring absorbance at 226 nm using an online
spectrophotometer. Fractions that showed antibacterial activity
were pooled and lyophilized.