, and large headspace volume significantly accel- erated the deterioration of tomato seed oil during storage, among which the temperature had the most pronounced effect followed by light and headspace volume. It is suggested that tomato seed oil is packaged with minimal light exposure and stored at a low temperature. The study provided important information for the quality evaluation and control of tomato seed oil and has a great significance for value-added utilization of the by-product from tomato processing industry.