retain their original flexibility. The printed filter paper samples
were then heated in an oven at 100 ◦ C for 8 min to cure alkenyl
ketene dimer onto the cellulose fibres. A stop watch, ruler, video
camera, and optical microscope were used for the characterization
of liquid penetration inside paper microfluidic channels.
Paper sheets with hydrophobized patterns were then printed
with detection reagents to form complete sensors. Two printed
chemistries were used to visually demonstrate the applications of
the sensors. First, a 1 mg/mL alkaline phosphatase enzyme solu-
tion was printed into the detection zone of a sensor. The BCIP® /NBT
liquid substrate was introduced into the sensor via its sample intro-
duction zone. The liquid substrate penetrated through the channel,
reaching the detection zone, and lead to the color change that
confirms the presence and the activity of the enzyme. Second, a col-
orimetric reaction for NO2 − detection was employed. An indicator
solution for colorimetric detection of NO2 − (an aqueous solution of
citric acid, sulfanilamide and N-(1-naphthyl)-ethylenediamine [9])
was printed onto the sheet with same patterns and into the detec-
tion zones of all sensors. Five microlitres of NO2 − sample solution
(5 mM) was manually introduced into the microfluidic channels
of all sensors and allowed to penetrate into the detection zones,
showing a color change.
retain their original flexibility. The printed filter paper sampleswere then heated in an oven at 100 ◦ C for 8 min to cure alkenylketene dimer onto the cellulose fibres. A stop watch, ruler, videocamera, and optical microscope were used for the characterizationof liquid penetration inside paper microfluidic channels. Paper sheets with hydrophobized patterns were then printedwith detection reagents to form complete sensors. Two printedchemistries were used to visually demonstrate the applications ofthe sensors. First, a 1 mg/mL alkaline phosphatase enzyme solu-tion was printed into the detection zone of a sensor. The BCIP® /NBTliquid substrate was introduced into the sensor via its sample intro-duction zone. The liquid substrate penetrated through the channel,reaching the detection zone, and lead to the color change thatconfirms the presence and the activity of the enzyme. Second, a col-orimetric reaction for NO2 − detection was employed. An indicatorsolution for colorimetric detection of NO2 − (an aqueous solution ofcitric acid, sulfanilamide and N-(1-naphthyl)-ethylenediamine [9])was printed onto the sheet with same patterns and into the detec-tion zones of all sensors. Five microlitres of NO2 − sample solution(5 mM) was manually introduced into the microfluidic channelsof all sensors and allowed to penetrate into the detection zones,showing a color change.
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