The conventional vegetative propagation of A. paniculata is too slow to meet the demand of pharmaceutical industries. Variability among the seed derived progenies and delayed rooting of seedlings restrains propagation through seeds [10]. Thus attempts were made by many laboratories to increase the quantity of andrographolide in A. paniculata plant parts using different inducers. Further to meet the overgrowing demand of andrographolide by pharmaceutical companies, attempts were also made to multiply A. paniculata through tissue culture. But callus developed on tissue culture media do not show detectable level of andrographolide. So the aim of the study was to treat the leaves of the plant with different growth regulator and to induce andrographolide production in the callus with the inducer that show best induction of andrographolide in leaves.