All HPLC analyses were accomplished with an Agilent 1200 liquid chromatography system (Agilent Technologies Deutschland,Waldbronn, Germany), equipped with a vacuum degasser, a quaternary
solvent delivery pump, a thermo-stated column compartment and a refractive index detector (RID). All HPLC analyses were carried on a Carbomix Ca-NP5: 8% column (7.8 300 mm,5 lm) at 80 C. Pure water was used as mobile phase in elution.The flow-rate was 0.3 ml/min. 30 ll of sample solution was injected for each HPLC analysis. For the analysis of authentic honey and syrup samples, each sample was analysed twice in triplicate.For the determination of linearity of peak height, six replicate analyses at each content level of syrup were performed. Finally, for the commercial samples inspection, all samples were analysed in triplicate at certain concentration.
All HPLC analyses were accomplished with an Agilent 1200 liquid chromatography system (Agilent Technologies Deutschland,Waldbronn, Germany), equipped with a vacuum degasser, a quaternarysolvent delivery pump, a thermo-stated column compartment and a refractive index detector (RID). All HPLC analyses were carried on a Carbomix Ca-NP5: 8% column (7.8 300 mm,5 lm) at 80 C. Pure water was used as mobile phase in elution.The flow-rate was 0.3 ml/min. 30 ll of sample solution was injected for each HPLC analysis. For the analysis of authentic honey and syrup samples, each sample was analysed twice in triplicate.For the determination of linearity of peak height, six replicate analyses at each content level of syrup were performed. Finally, for the commercial samples inspection, all samples were analysed in triplicate at certain concentration.
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