4. Fill another sample cell with 10 mL of sample (the prepared sample).
5. Add the contents of one Ascorbic Acid Powder Pillow to each cell. Swirl to mix.
6. Add 12 drops of Alkaline-Cyanide Reagent Solution to each cell. Swirl to mix.
Note: A cloudy solution may form in some samples after reagent addition. The turbidity should dissipate after Step 8.
Note: A Tensette Pipet may be used to dispense 0.4 mL of the Alkaline Cyanide Reagent.
7. Add 12 drops of PAN Indicator Solution, 0.1%, to each sample cell. Swirl to mix.
Note: An orange color willdevelop in the sample if manganese is present.
Note: A Tensette Pipet may be used to dispense 0.4 mL of the PAN Indicator Solution.