The culture supernatant was removed from the tube containing
nutrient broth by repeated centrifugation. Exactly 500 μl of bacterial
suspension ca. 1108 cfu/ml was taken in a multi-dish
containing a small block of P. capsici mycelia and then they were
kept in dark for 3 days. After three days, the multi-dishes were
kept for 20 min at 0 °C for cold-shock and then allowed to stand
for 1 h at room temperature for zoospore release