An efficient in vitro propagation protocol has been developed for P. foetida in the present study. A very good number of shoots were multiplied and almost all the shoots were rooted using ex vitro method. It reduced time, energy and cost of production of micropropagated plantlets and increased the chances of survival in the field condition. This protocol can be used for mass scale production of superior and disease free plants which could be replanted in the forest area and supplied to the farmers at reduced cost as standard stock planting material.