ii. By ultrasonication. Ultrasound-assisted solvent extraction was
performed in an ultrasonic bath (Barson, USA) at ambient temperature
(25°C) for 3 h by keeping the plant material (10.0 g) in methanol
(100.0 mL). The methanol extract was filtered and evaporated to dryness
under reduced pressure on a rotary evaporator and was dried to yield the
sample free of solvent.