LAB isolates were cultivated in MRS
for 16-18 h with 1% inoculum then cells were removed from
MRS medium by centrifugation (6500g for 10 min, 4 C) to
obtain cell free supernatant. Lawns of pathogenic strains were
prepared by adding 0.125 ml (2 107 cell/ml) of 10 diluted
overnight culture to 5 ml of corresponding soft agar (Table 2).