To evaluate the effectiveness of inoculants addition in a bioremediation
process, seawater microcosms simulating a crude oil spill were prepared.
Seawater was collected in sterile glass bottles from the Main Channel of
Bahı´a Blanca Estuary (381 440–391 270 S; 611 450–621 300 W).The bottles
were transported to the laboratory at 10 1C and stored at 4 1C for no more
than 24 h. Twelve sterile 250mL Erlenmeyer flasks were prepared with
150mL of seawater and 0.75mL of autoclaved crude oil (type:
‘‘Medanito’’; origin: Neuque´n, Argentina; density: 0.84), provided by a
local refinery