plates of YEPD were inoculated onto the surface with
3 mL of a diluted YEPD overlay (0.5% yeast extract, 1% peptone,
1% dextrose, and 0.6% agar) containing 0.1 mL of S. reilianum, and
cultivated for 18 h at 28 C and 180 rpm. Then B. subtilis was sown
with a sterile stick. The plates were incubated at 28 C for 72 h and
observed every 12 h. The B. subtilis strains that showed the capacity
to inhibit proliferation of the fungus were selected.