The solid residues obtained after steam explosion were subject
to enzymatic saccharification. The substrate consistency is 10% (w/
v). Two commercial enzymes, cellulase (Celluclast 1.5 l, Cat C2730)
and b-glucosidase (Novozyme 188, Cat C6105), were used. The
hydrolysis experiments were conducted in 50 mM citric acid buffer
(pH 4.8) at 50 C and 170 rpm for 48 h. The dosage of cellulase and
b-glucosidase were 15 filter paper units (FPU) and 30 cellobiase
unit (CBU) per gram residues, respectively. The resultant hydrolyzates
were then isolated by centrifugation at 7000 rpm, and the
supernatant was utilized as a medium component.