All samples were screened for GMO content with the Foodproof
GMO Screening Kit, according to the manufacturer's instructions,
using 80 ng of sample DNA in a final reaction volume of 10 m l. A
real-time PCR protocol for the LightCycler 480 II was set up as
described previously (Turkec, Kazan, Baykut, & Lucas, 2015) with
multi-color detection in 4 channels (FAM; VIC/HEX; Rox; Cy5). A
color compensation object was first created using Color Compen-
sation Set 3 (Biotecon Gmbh, Potsdam, Germany) and all samples
were then screened at least twice on different days, alongside
appropriate CRMs as controls.