4. Discussion
The optimal medium for C. nutans propagation
was MS + 1.0 mg L-1 BA + 0.02 mg L-1 NAA, which
provided a multiplication rate of 3.9 shoots per passage during passages of 30-d duration. Annual shoot
production was, therefore, approximately
3.912=12,381,557, which allows mass-propagation of C.
nutans in a tissue culture facility. The shoot
multiplication rate increases, but shoot size diminishes,
during long-term production possibly due to cytokinin
accumulation. Thus, BA and NAA concentrations
could be adjusted during long-term propagation to
maximize shoot production and quality (Chen et al,
2014).