The
flow system was based on: a proportional injector made in acrylic;
polypropylene tubes, each with internal diameter of 0.8 mm; a
IPC4 Ismatec peristaltic pump furnished with Tygon pumping
tube; two T-type joint devices machined in acrylic; a thermostatic
bath (Tecnal, model TE 184); spectrophotometer (Femto, model
700) equipped with a flow cell (ca. 180 lL) with optical path of
10 mm.