Carotenoid extractiMethod 1. Magnesium hydroxide carbonate to neutralize any organic acids and internal standard (-apo-8-carotenal for fruits or echinenone for green vegetables), were added to each analytical sample (2 g, in triplicate), followed by four repeated
extractions, each time with 50mL of a 1:1 (v/v), THF and MeOH mixture, using a DI 25 basic batch high-performance homogeniser(IKA-WERKA GMBH) at 13,500rpm during 1min followed by
vacuum-filtration.on from foods.