not different from literature values (Walker and others 2012). The yolk lipid content calculated from the GC-FAME analysis was about 77% of the total lipids for the 5 diets. The presence of high percentage of phospholipids in yolk lipids explains the difference from the 100% value because the polar functional groups on phos- pholipids were not converted to methyl esters (Walker and others
2012).
Unsaponifiable matters of egg yolk lipids of the 5 diet treatments were about the same, 1.1% of dry yolk lipids on average (Table 5). Unsaponifiable matters include carotenoids, cholesterol, vitamin D, and other sterols, of which cholesterol is the major component. Elevated cholecalciferol content in egg yolk did not significantly increase the concentration of unsaponifables in the egg yolk.