For Bradford’s assay (Bradford, 1976), a calibration curve up to 1mgmL1 of BSA was prepared in the extraction solvent (water:- ACN mixture (7:3, v/v). Sample protein amount was measured according to the protocol described in the Bradford assay Protein Quantitation Kit-Rapid (Fluka). A sample blank containing 5% (v/v) Celluclast 1.5L enzyme was also made in order to remove its contribution to the final sample absorbance.