Curcumin labeling of amyloid deposits
Ex vivo treatment of paraformaldehyde fixed brain tissue sections from APP mouse with curcumin showed bright green fluorescent staining after a 20-min incubation. Curcumin staining was histochemically confirmed with thioflavin S, as shown in Fig. 1 (panels a and b). Ex vivo staining showed that all thioflavin S-positive plaques were also curcumin positive. Ex vivo staining with methoxy-XO4 also showed co-localization, regardless of whether curcumin was added before or after methoxy-XO4, demonstrating that the two compounds do not compete for binding to senile plaques.