Phyllanthus emblica (Euphorbiaceae) possesses a
vast ethnomedical history and represents a phytochemical
reservoir of heuristic medicinal value. Polyphenolic
extract of P. emblica fruits, was investigated
for antioxidant potential against lead acetate induced
toxicity in female Wistar rats. The liver of female
Wistar rats treated with lead acetate at different concentrations
showed significant increase in lipid peroxidation
and decrease in activities of superoxide
dismutase and catalase. Furthermore, lead acetate
also caused decrease in reduced glutathione content
in rat liver. However, the co-incubation of extract
and lead acetate showed significant protection in
concentration-dependent manner. Furthermore, the
polyphenolic extract of P. emblica fruits also found
to have potent free radical scavenging activity as
assessed by reducing power assay, 2,2-diphenyl-1-
picrylhydrazyl (DPPH) radical (IC50 15.5 μg/mL), superoxide
radical (IC50 26 μg/mL) and hydrogen peroxide
scavenging assays (IC50 22 μg/mL) under in vitro conditions.
Moreover the phytochemical characterization
of the extract was also measured by determining
total phenolic, flavonoids and tannin contents. This
activity of the extract may be due to the total polyphenolic
contents present in it. The present study
concluded that a polyphenolic rich fraction of P.
emblica fruits is a potential source of natural antioxidants
and thus could prevent oxidative stress induced
by heavy metal toxicants.
Phyllanthus emblica (Euphorbiaceae) possesses avast ethnomedical history and represents a phytochemicalreservoir of heuristic medicinal value. Polyphenolicextract of P. emblica fruits, was investigatedfor antioxidant potential against lead acetate inducedtoxicity in female Wistar rats. The liver of femaleWistar rats treated with lead acetate at different concentrationsshowed significant increase in lipid peroxidationand decrease in activities of superoxidedismutase and catalase. Furthermore, lead acetatealso caused decrease in reduced glutathione contentin rat liver. However, the co-incubation of extractand lead acetate showed significant protection inconcentration-dependent manner. Furthermore, thepolyphenolic extract of P. emblica fruits also foundto have potent free radical scavenging activity asassessed by reducing power assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (IC50 15.5 μg/mL), superoxideradical (IC50 26 μg/mL) and hydrogen peroxidescavenging assays (IC50 22 μg/mL) under in vitro conditions.Moreover the phytochemical characterizationof the extract was also measured by determiningtotal phenolic, flavonoids and tannin contents. Thisactivity of the extract may be due to the total polyphenoliccontents present in it. The present studyconcluded that a polyphenolic rich fraction of P.emblica fruits is a potential source of natural antioxidantsand thus could prevent oxidative stress inducedby heavy metal toxicants.
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