1) A Phase contrast microscope is necessary, 2) Cells with low densities may be difficult. 3) Motile cells may be difficult to count, 4) Debris can be mistaken for Microorganisms
1) A Phase contrast microscope is necessary, 2) Cells with low densities may be difficult.3) Motile cells may be difficult to count,4) Debris can be mistaken for Microorganisms