The coffee husk substrate was obtained from the Incofex Coffee
Corporation, in Viçosa, Minas Gerais State, Brazil. The husk was
boiled in water for 2 h, in order to reduce some compounds which
could inhibit fungal growth and contaminants, and centrifuged at
1800 rpm for 5 min to remove excess water. Next, 1.5 kg of each
sample were placed in polypropylene bags and autoclaved for
2 h. This procedure was repeated three times at 48-h intervals.
The final humidity was 80%. At the end of the procedure, once
the substrate reached room temperature, the inoculation with fungus
spawn was performed