2.5. Statistical analyses
The AWDA were conducted in triplicate. Data obtained were analyzed by a variance analysis (ANOVA), followed by Tukey's multiple comparison test. Data were processed using the statistical program Statgraphics (Statgraphics Plus for Windows, version 5.1, 2001, Manugistics, Inc., Rockville, Maryland, USA). The significance level was 0.05%.
Regarding to microdilution method, each mixture of antimicrobials was tested by quintuplicate, and the same results were found for each replicate.
DSC and PCR analysis were conducted in duplicate. Same results were obtained in each trial.