Mycoplasmal DNA was detected by qPCR in the right and left lung of all Hillcrest-challenged pigs at 21 and 28 days post-inoculation and in the spleen, kidney and liver of the majority of Hillcrest-challenged pigs at day 21. Fewer samples from abdominal sites of Hillcrest-challenged pigs were qPCRpositive at 28 days post-inoculation. Lung samples from 3 pigs inoculated with the Beaufort strain were qPCRpositive at 28 days post-inoculation.