This level was similar to the LODs of previous studies
done on other authentication assays. For the determination of
the LODr, mixtures were tested containing 2%, 1%, 0.1%, 0.01%
and 0.001% of S. salar L. (w/w), respectively. GH1 region was amplified
and sequenced successfully in all cases. The proposed method
meets the 0.01% sensitivity criterion for pooled samples. The
in-house LODs had a false-negative rate of 65% and a RSD of reproducibility
of 33%, which are both within ISO 24276 requirements
(ISO 24276:2006).