The column was further washed with 2 ml of isopropanol. Following centrifugation of the column at 12,000 g for 5 min, the DNA was eluted with 50 ml of pre-warmed (65 C) elution buffer (10 mM TriseOH).
The column was further washed with 2 ml of isopropanol. Followingcentrifugation of the column at 12,000 g for 5 min, the DNA waseluted with 50 ml of pre-warmed (65 C) elution buffer (10 mMTriseOH).