B. Pathogenic and Trichoderma Fungi Inoculants
Both Fusarium spp. was subcultured on Potato
Dextrose Agar at 25± 1°C. 10 ml of each of Fusarium
spp. culture suspension (107cfu/ ml) was added to soil
of each field. Trichoderma species were used: T.
harzianum (T-100) T. asperellum and T. viride.
Cultures were maintained on PDA medium and stored
at 4°C for further use. Concerning to the Trichoderma,
three types of treatments were used alone or in
combination; so that soil treatment by adding 10 mL from all or each of Trichoderma spp. for selected
tomato fields from a concentration of 108cfu/ ml were
added one week before Fusarium inoculation.