Conclusion
In conclusion, the present study reported micropropagation
results in D. aqueum for the first time. Individual auxins,
natural supplements and cytokinins represented effective
growth promoting factors. Efficient shoot regeneration, multiplication
and rooting by using individual treatments of auxins,
natural additives and cytokinins were established from protocorm.
Individual levels of NAA and IBA alone successfully
produced healthy shoots and roots both in multiple numbers
and in length than any other cytokinins from protocorms of
D. aqueum. Results obtained on natural additives BP and
CW are also noticeable in view of cost effective approach.
The efficient production of protocorms and subsequent
elongated multiple shoots and well rooted plants by individual
auxins and organic additives provides a simple and cost
effective protocol for mass propagation and conservation for
this species and could be tried to valuable Dendrobium species.