seven batches in medium E, the culture was reverted to medium C
to allow biomass growth under less stressful conditions. The adaptation
protocol was resumed, changing to media D and E after four
successive batches in each medium. Biomass concentration
increased slightly in the last batches made in medium D, but descended
in subsequent batches in medium E. Glucose exhibited high
variability, probably due to dilution by broth exchange, detection of
additional reducing carbohydrates in the medium and possible
interference by EDTA on DNS method.