extracted from leaves of Lady-Rosetta infected with both P.
infestans and A. solani yielded only the predicted PCR
product (813 bp) after 12 hpi. No PCR products were
obtained from plants un-inoculated with P. infestans (Fig.
10). On the other hand, using DNA templates prepared from
tuber-discs infected with a mixture of P. infestans and E.
carotovora or R. solanacearum, the primer detected fungal
DNA in samples inoculated with both P. infestans and R.
solanacearum only 48 hpi