D1 Transfer to genebank
Four to six weeks following multiplication, fully developed seedlings (i.e., seedling showing some roots and at least 2 nodes) are transferred to the genebank (Fig. 16). Genebank culture conditions are as follow: 18–20oC and a 12/12 photoperiod
Germplasm is observed weekly in order to eliminate and record any contaminated (Fig. 17) or necrosed (Fig. 18) seedlings.