A most probable number method (National Food Hygienic Standard
of China 4789.3‐2010) was used to detect the coliform bacteria in strawberry
juices. In the pre-fermentation test, samples were diluted into
three appropriate series, and 1.0 mL sample was inoculated in triplicate
tubes of 10 mL lauryl sulfate tryptose (LST) broth (Beijing Luqiao Biological
Technology Co. Ltd., Beijing, China). Inoculated tubeswere incubated
at 37 °C for 48±2 h. And then, in the re-fermentation test, loopfuls of
samples from inoculated LST broth, which were positive for turbidity
and gas formation, were inoculated in tubes of 10 mL of brilliant green
lactose bile (BGLB) broth (Beijing Luqiao Biological Technology Co. Ltd.,
Beijing, China). After they were incubated at 37 °C for 48±2 h, the positive
tubes with color and turbidity changes and gas formation were
counted.