IDF participated more in these interactions, especially at 100 C. However, no definite correlation was established between the nitrogen content of the fiber and extrusion conditions. It should be noted that such protein-fiber interactions would not have contributed to increased dietary fiber content of the flour (Table 3)because appropriate deductions were made for protein content (calculated from the crude nitrogen content during the quantification of IDF and SDF). However,the fiber–protein complexes present in extruded barley may resist digestion by enzymes in the human intestine and pass on to the colon, along with dietary fiber. The fate of this protein–fiber complex in the colon or its benefit to human health is yet to be determined.