The wild-type strain was stored on a potato dextrose agar (PDA) (Nissui
Pharmaceutical Co., Ltd., Japan) slant, and the mutant was on an agar slant of
Vogel’s medium [39] supplemented with 200 mM of 8-azaguanine at 5 ◦C. Prior to
the experiment, a piece of mycelia in the stock culture was transferred to a fresh
PDA slant and incubated at 25 ◦C for eight days