Yeast isolation and characterization
Two yeast strains, SAM09T and SAM10, were isolated from
some flowers of a single halophytic semi-shrub (Salsola sp.)
collected from a desert in East Azerbaijan Province, Iran in October 2012. To isolate the strains, the flowers were
washed with sterile distilled water, shredded, and macerated
in 20 ml sterile saline solution for 30 min on a shaker.
Aliquots of 100 ml from the suspension were spread on the
surface of YM agar (0.3% yeast extract, 0.3% malt extract,
0.5% peptone, 1.0% glucose and 2.0% agar; Kurtzman
et al., 2011) supplemented with 0.01% chloramphenicol.
After incubation at 28 uC for 7 days, yeast colonies were
isolated and purified for later identification.