conclusion, this report describes a two step culture protocol for large scale multiple shoot bud regeneration from axillary nodalexplants of G. sylvestre. The maximum shoot bud induction (84.2%)as well as shoot number (14.2) without callus formation occurredon MS medium augmented with BAP and KIN in the first step,whereas, the combination of BAP (1.0 mg/l) + KIN (0.5 mg/l) + GA3(1.0 mg/l) produced 418.7 shoots/explant after 5th subculture inthe second step. The in vitro regeneration protocol described hereis different from that of G. sylvestre reported earlier. Thus the cul-ture of shoot clumps in the presence of GA3containing mediumincreased the number of multiple shoots in Gymnema. The presentstudy