Reference strains and environmental isolates
were subcultured on Columbia agar base (Oxoid, product
code CM0331) for testing for acid phosphatase, whilst the
environmental isolates were also tested for motility,
reduction of nitrate, fermentation of lactose and liquefaction
of gelatin using buffered nitrate motility and lactosegelatin
media according to the UK reference method
(Anon. 2004).