One half of the root system was planted in
a separate pot and transferred to a climate chamber. Each half of
the plant root was artificially wounded and inoculated sequentially,
4 and 6 days after transplanting with 40 mL and 20 mL
antagonistic and R. solanacearum suspensions, respectively.Each
plant was monitored and disease symptom development was recorded. Five days post inoculation, quantification of the pathogen
population in mid-stems was performed as described above.