Extracellular LiP, MnP and Laccase activities by C. globosum IMA1 C. globosum was able to grow on ITE, characterized by a COD of 2.3 g/L and equivalent indigo concentration of 0.35 g/L. It produced LiP, MnP and Laccase with high activities in both static and stirred cultures (Fig. 5). The LiP, MnP and Laccase activities were significantly favored by agitation with maximum values of 832 U/L (Fig. 5a), 250 U/L (Fig. 5b) and 83.4 U/L. Agitation increased the oxygen and nutrient transfer rate which was decisive for biomass growth and enzymes production and activities. Therefore, the high ITE decolourization was corroborated with the extracellular peroxidases and mycelium growth, which favor the biodegradation of complex molecules. The time courses of LiP and MnP production by C. globosum in both static and stirred conditions showed highest activities in 3 days and 5 days of incubation, respectively and then decreased. However, the Laccase enzyme was measured in the supernatant until the 8th hour of culture with a maximum activity in 3 days.