The present study constitutes the first report on the micropropagation of self-heading cultivars of Philodendron. Results in the present study demonstrated that the stem nodal segments showed a higher potential to produce shoot than the leaf lamina and petiole explants under the treatment conditions tested. Shoots can be induced to form following the 0.5 and 1 mg l−1 of BA treatments at frequencies of 55.6–80.6% and with an average shoot number of 40.8–50.4 per explant depending on the cultivar. The induced shoots could elongate on PGR-free MS medium to produce normal looking shoots, and rooted successfully on IBA-contained media. Following transfer to the greenhouse conditions, 100% of the rooted shoots survived. The procedure developed in the present study can be easily implemented for the mass production of Philodendron on a commercial level.