VCO was prepared according to the method reported by Hamid
et al. (2011) with slight modification. The fresh endosperm of
mature coconut was grated and made into viscous slurry and then
squeezed using a cheese cloth to obtain coconut milk. The coconut
milk was chilled to 10 C to break the emulsion and then centrifuged
at 830g for 5 min to obtain raw coconut cream, skim milk,
and some proteins or solid components. The coconut cream was
kept at 4 C for 24 h and then subjected to mild heating (65 C)
in a thermostat oven until the cream temperature reached 65 C,
followed by centrifugation (2306g for 10 min) to separate non-oil
fractions from the oil phase. Finally, the raw oil was filtered
through Whatman filter paper to obtain VCO. The obtained VCO
was labeled VCO-1 and used for the adulteration study