Effect of botanicals on incidence and severity of mango fruit anthracnose: One hundred and fifty green matured mango fruits were randomly collected from the sampling areas. The fruits were mixed together and later subdivided into 10 parts of 15 fruits each with each fruit representing a replicate. Each part containing 15 numbered fruits was treated separately with botanicals (water or alcohol extraction) and fungicide (benomyl). One part was treated with sterile distilled water to serve as control.
For botanicals, the fruits were soaked in aqueous or alcohol plant extract for 30 min and air-dried. Benomyl 50% w/w at 100 g/100 L of water was sprayed over the fruits and completely covered with sterilized paper towel while in the case of control treatment; the fruits were thoroughly washed and soaked in sterile distilled water for 30 min and air dried.
The treated fruits for each treatment were arranged in separate boxes laid out in a completely randomized design layout and allowed to ripen for 16 days under humid condition. The fruits were individually examined for anthracnose lesions every other day beginning from the sixth day after treatment when ripening has commenced until the sixteenth day. Anthracnose incidence and severity was assessed and calculated as described above.