We were able to isolate a biotechnologically important yeast-like fungus from a biogas reactor. This new strain of A. pullulans
was able to produce a high level of EPS in comparison with other
tropical A. pullulans isolates. The concentration of the nitrogen in
the fermentation medium affects microbial biomass accumulation
and the yield of pullulan produced by A. pullulans. The study has
also confirmed that the concentration of EPS produced by A. pullulans was dependent on the carbon and nitrogen sources. Further
work onmediumand process optimization for EPS production is in
progress.