For the cellulose, control values of 0.52, 1.20, 0.20, and 1.46 U ml−1, and SCB values of 0.95, 1.60, 0.21,and 1.49 U ml−1 were obtained, respectively. Although the enzymatic activities of the culture with biologically pretreated sugar cane bagasse were lower than the cultures carried out with untreated sugar cane bagasse, it should be noted that production of enzymes of the cellulase and hemicellulase complex after production of the mushrooms is another way to add value to this agricultural residue.