The MS2 fragmentation of aglycone side chain produces the most
consistent ion at m/z 259, and the MS3 fragmentation of this
ion gives rise to fragments at m/z 97 (corresponding to the
sulfate group) by the disassociation of GLSs in the ion trap mass
spectrometer, constituting a very useful preliminary screening
method for determining the presence of GLS in sprouts
extracts