Non-irradiated and irradiated cherry tomatoes (25 g) were placed in sterile stomacher bags containing 100 mL of 0.1% Tween 80 physiological solution. Samples (n = 9/dose) were homogenized using a stomacher (Stomacher 3500; Seaward, UK) for 15 min. Serial decimal dilutions were prepared for inoculation on Tryptic Soy Agar plates (TSA) (Oxoid LTD, Basingstoke, England) for bacterial counts and Malt Extract Agar plates (MEA) (Merck KGaA, Darmstadt, Germany) for filamentous fungi counts. Samples were incubated at 30 °C for TSA plates and 28 °C for MEA plates and colony numbers were counted for 7 days. The results were expressed as log colony-forming units (CFU) per gram of fresh fruit.