7. Organosolv
In this method, organic solvents such as ethanol, methanol, acetone, and ethylene glycol or their mixture with water are used to remove lignin and hydrolyze hemicellulose, leading to the improved enzymatic degradability of cellulose [22]. However, solvents should be removed before fermentation, which is a costly process. The temperature of the process is about 200 °C or less, depending on the type of biomass and catalyst. High-quality lignin is produced in organosolv pretreatment, which can be used for production of chemicals. The removal of lignin decreases the cost of enzyme because the absorption of cellulase to lignin is decreased. In this method formation of inhibitors of fermentation is very low although this method is applicable to various biomasses with a high potential of sugar yield [6].
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7. Organosolv In this method, organic solvents such as ethanol, methanol, acetone, and ethylene glycol or their mixture with water are used to remove lignin and hydrolyze hemicellulose, leading to the improved enzymatic degradability of cellulose [22]. However, solvents should be removed before fermentation, which is a costly process. The temperature of the process is about 200 °C or less, depending on the type of biomass and catalyst. High-quality lignin is produced in organosolv pretreatment, which can be used for production of chemicals. The removal of lignin decreases the cost of enzyme because the absorption of cellulase to lignin is decreased. In this method formation of inhibitors of fermentation is very low although this method is applicable to various biomasses with a high potential of sugar yield [6]. 8
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