2.9. Transmission electron microscopy (TEM)
The morphology of WSH30 and WSH40 were examined by TEM
in a Zeiss EM 109 transmission electron microscope equipped with
a system for digital image capture Olympus MegaView V. A drop
of diluted nanocrystals aqueous suspension was deposited on a Cu
microgrid (200 mesh) and allowed to dry. The grid was negatively
stained with a 3%(w/w) solution of uranyl acetate and dried at room
temperature. The sample was observed at 80 kV. The dimensions of
whiskers were determined using digital image analyses (ImageJ).
A hundred nanocrystals were randomly selected and a minimum
of 100 and 200 measurements were used to determine the average
length and the diameter, respectively