2.3. Foreground and Recombinant Selection. At the initial stages of the experiment, for selection of the Saltol locus (foreground), the reported rice microsatellite (RM) markers
RM493 and RM3412b, which were found to be tightly linked to Saltol, were used for foreground selection. For franking markers used for recombinant selection, about 5Mb region of the Saltol region was targeted. Four polymorphic microsatellite markers (RM1287, RM10694, RM562, and
RM7075) were identified for recombinant selection (Table 1, Figure 2).